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目的:测定日本蛇根草不同组织器官及发育周期中绿原酸的含量。方法:建立高效液相色谱法。色谱柱ODS Phenomenex Gemini C18柱(250 mm×4.6 mm,5μm),流动相乙腈:0.4%磷酸溶液(1∶9),流速:1.0 ml·min-1,检测波长:327 nm;柱温:30℃。结果:绿原酸在0.022~1.320μg(r=0.999 9)范围内线性关系良好。2月花期采收的日本蛇根草根、茎、叶、花中绿原酸的含量分别为7.076 7,1.030 7,10.609 5,24.110 3 mg·g-1,其全草与四月果期的全草绿原酸含量分别为18.600 4,14.082 6 mg·g-1。结论:日本蛇根草不同组织器官绿原酸含量差异较大,其中花的含量最高。不同发育周期绿原酸的含量也不同,花期含量明显高于果期。
Objective: To determine the content of chlorogenic acid in different tissues and organs of Japanese snakehead grass and its development cycle. Methods: To establish high performance liquid chromatography. Column: ODS Phenomenex Gemini C18 column (250 mm × 4.6 mm, 5 μm), mobile phase acetonitrile: 0.4% phosphoric acid solution (1:9), flow rate: 1.0 ml · min -1, detection wavelength: 327 nm, column temperature: 30 ℃. Results: Chlorogenic acid showed good linearity in the range of 0.022-1.320 μg (r = 0.999 9). The contents of chlorogenic acid in roots, stems, leaves and flowers of Japanese snakehead roots harvested in February were 7.076 7, 1.030 7, 10.609 5 and 24.110 3 mg · g -1, respectively, Whole grass chlorogenic acid content were 18.600 4,14.082 6 mg · g-1. Conclusion: The contents of chlorogenic acid in different tissues and organs of Japanese snakehead grass vary greatly, and the content of flowers is the highest. The content of chlorogenic acid in different developmental cycles is also different, the content of florescence is obviously higher than that of fruit.