Two acetate-fed sequencing batch reactors (SBR) were operated under an aerobic dynamic feeding (ADF) model (SBR#2) and with anaerobic phase before aerobic phase (SBR#1) to select mixed cultures with a high polyhydroxyalkanoates (PHA) storage response.Although kinetic selection based on storage response should bring about a predominance of floc-formers,a bulking sludge with storage response comparable to well-settled sludge was steadily established.An anaerobic phase was introduced before the aerobic phase in the ADF model to improve the sludge settleability (SBR #1),however,due to the consequent increased feast/famine ratio,the performance of SBR #1,in terms of both the maximum PHB (polyhydroxybutyrate) cell content and △PHB,was lower than that of SBR #2.SBR #2 gradually reached a steady state while SBR #1 failed suddenly after 50 days of operation.The maximum specific substrate uptake rate and storage rate for the selected bulking sludge were 0.4 Cmol Ac/(Cmol X.hr) and 0.18 Cmol Ac/(Cmol PHB.hr),respectively,resulting a yield of 0.45 Cmol PHB/(Cmol Ac) in SBR #2 in the culture enrichment phase.A maximum PHB content of 53％ of total suspended solids and PHB storage rate of 1.36 Cmol Ac/(Cmol PHB.hr) was achieved at 10.2 hr in batch accumulation tests under nitrogen starvation.The results indicated that it was feasible to utilize filamentous bacteria to accumulate PHA with a rate comparable to well-settled sludge.Furthermore,the lower dissolved oxygen demand of filamentous bacteria would save energy required for aeration in the culture enrichment stage.