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目的 :建立用反相高效液相色谱法同时测定古方生化汤中阿魏酸和川芎嗪含量的方法。方法 :以甲醇 水 (含 1%的冰醋酸 ) ,(7∶18)为流动相 ,ZorbaxC18柱 (2 5 0mm× 4 .6mm ,5 μm)为固定相 ,紫外检测波长为 2 80nm ,外标法定量。 结果 :阿魏酸和川芎嗪分离完全 ,阿魏酸在 8.0 6～ 6 4 .4 8mg·L-1的浓度范围内回归 ,方程为Y =1811.32 +1170 .33X ,r =0 .995 4 ,平均回收率为 97.9% ,RSD为 1.4 % ;川芎嗪在 4 .0 3～ 2 0 .15mg·L-1的浓度范围内 ,回归方程为Y =16 .6 0 +14 0 1.94X ,r =0 .994 1,平均回收率为 10 1.9% ,RSD为 2 .6 %。结论 :此法快捷、简便 ,准确 ,可作为生化汤的质量标准。目的 :建立用反相高效液相色谱法同时测定古方生化汤中阿魏酸和川芎嗪含量的方法。方法 :以甲醇 水 (含 1%的冰醋酸 ) ,(7∶18)为流动相 ,ZorbaxC18柱 (2 5 0mm× 4 .6mm ,5 μm)为固定相 ,紫外检测波长为 2 80nm ,外标法定量。 结果 :阿魏酸和川芎嗪分离完全 ,阿魏酸在 8.0 6～ 6 4 .4 8mg·L-1的浓度范围内回归 ,方程为Y =1811.32 +1170 .33X ,r =0 .995 4 ,平均回收率为 97.9% ,RSD为 1.4 % ;川芎嗪在 4 .0 3～ 2 0 .15mg·L-1的浓度范围内 ,回归方程为Y =16 .6 0 +14 0 1.94X ,r =0 .994 1,平均回收率为 10 1.9%
Objective : To establish a method for simultaneous determination of ferulic acid and tetramethylpyrazine in Gufang Shenghua Decoction by reversed phase high performance liquid chromatography. Methods: The mobile phase consisted of methanol water (containing 1% glacial acetic acid), (7:18) as mobile phase, Zorbax C18 column (250mm×4.6mm, 5μm) as stationary phase, UV detection wavelength of 280nm, external standard Quantitative method. RESULTS: Ferulic acid and ligustrazine were completely separated, and ferulic acid was returned within a concentration range of 8.0 6 648.4 mg·L-1. The equation was Y=1811.32 +1170.33X ,r =0.995 4 , The average recovery was 97.9% and the RSD was 1.4%. The concentration of tetramethylpyrazine was in the range of 4.03 to 20.15mg·L-1. The regression equation was Y=16.60 +14 0 1.94X, r = 0 .994 1, the average recovery was 10 1.9%, RSD was 2.6%. Conclusion: This method is fast, simple, accurate and can be used as the quality standard of biochemical soup. Objective : To establish a method for simultaneous determination of ferulic acid and tetramethylpyrazine in Gufang Shenghua Decoction by reversed phase high performance liquid chromatography. Methods: The mobile phase consisted of methanol water (containing 1% glacial acetic acid), (7:18) as mobile phase, Zorbax C18 column (250mm×4.6mm, 5μm) as stationary phase, UV detection wavelength of 280nm, external standard Quantitative method. RESULTS: Ferulic acid and ligustrazine were completely separated, and ferulic acid was regressed within a concentration range of 8.0 6 648.4 mg·L-1. The equation was Y = 1831.32 +1170.33X, r = 0.995 4 . The average recovery was 97.9% and the RSD was 1.4%. The concentration of tetramethylpyrazine was in the range of 4.03 to 20.15mg·L-1. The regression equation was Y=16.60 +14 0 1.94X, r = 0 .994 1, the average recovery rate was 10 1.9%