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目的建立商陆中商陆皂苷甲的HPLC-UV测定方法。方法用Alltima C18色谱柱(4.6 mm×250 mm,5μm),流动相:乙腈-0.1%磷酸水=40∶60,流速:0.8 m L·min-1,检测波长:203 nm,考察该方法的专属性、标准曲线和定量下限、精密度与回收率、稳定性以及重现性。结果商陆皂苷甲在0.15~3.86μg内,线性关系良好,定量下限0.039μg,精密度良好(日内、日间RSD分别为1.12%和1.89%),平均回收率为98.61%。10个不同产地样品中商陆皂苷甲的含量为0.05%~0.42%。结论本方法简单,重复性好,适合用于商陆药材的质量控制。
OBJECTIVE To establish HPLC-UV method for the determination of meconoside A in commercial land. Methods Alltima C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was acetonitrile-0.1% phosphoric acid water 40:60, the flow rate was 0.8 m L · min-1 and the detection wavelength was 203 nm. Specificity, standard curve and lower limit of quantitation, precision and recovery, stability and reproducibility. The results showed that within the range of 0.15-3.86μg, the esculentoside A had a good linearity. The lower limit of quantification was 0.039μg. The precision was good (intraday RSD and intraday RSD were 1.12% and 1.89%, respectively). The average recovery was 98.61%. The contents of esrinsidium saponin in 10 samples of different origins ranged from 0.05% to 0.42%. Conclusion The method is simple, reproducible, suitable for quality control of medicinal land.