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目的分析新疆乌鲁木齐市2008年流行的11株成人麻疹野病毒的基因特征。方法用Vero/slam细胞从成人麻疹暴发患者的标本中分离出麻疹野病毒,应用逆转录一聚合酶链反应(RT-PCR)对麻疹野病毒分离株扩增核蛋白N基因羧基末端676个核苷酸片段,再对扩增产物进行核苷酸序列测定和分析。并以N基因羧基末端450个核苷酸片段构建基因亲缘性关系树,进行核苷酸变异分析。结果共分离到1l株麻疹野病毒,这些病毒N基因450个核苷酸之间的差异为0%～0.2%,均为H1基因型中的Hla基因亚型。11株麻疹野病毒与H1基因型代表株(Chin9372)的核苷酸和氨基酸差异分别为2.2%～2.4%之间和4.0%~4.6%之间。结论 2008年新疆乌鲁木齐流行的成人麻疹野病毒为Hla基因亚型。
Objective To analyze the genetic characteristics of 11 adult wild measles wild viruses that were prevalent in Urumqi, Xinjiang in 2008. Methods Vero / slam cells were isolated from specimens of adult measles outbreaks and wild-type measles virus was amplified by RT-PCR from 676 nucleotides Nucleotide fragment, and then the amplified product was subjected to nucleotide sequence determination and analysis. Nucleotide sequence analysis was carried out by constructing a phylogenetic tree of 450 nucleotides at the carboxyl terminus of N gene. Results A total of 11 strains of wild measles virus were isolated. The nucleotide variation of 450 nucleotides in these N genes was 0% -0.2%, all of which were Hla subtypes in H1 genotype. The nucleotide and amino acid differences between the 11 measles wild-type virus and the H1 genotype representative strain (Chin9372) were between 2.2% -2.4% and 4.0% -4.6%, respectively. Conclusion In 2008, the adult wild measles virus in Urumqi was Hla subtype.