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目的：建立了ＨＰＬＣ法测定血清样品中伊曲康唑的定量分析方法。方法：色谱条件：以ＺＯＲＢＡＸＴＭＣ１８（５μｍ，４．６×１５０ｍｍ）为色谱柱；乙腈水（６７．５∶３２．５）为流动相，检测波长２６３ｎｍ；用正庚烷∶异戊醇（９８．５∶１．５）作为提取剂。结果：三种浓度４０，８０，３００ｎｇ·ｍｌ－１回收率分别为１０５．０５％，１００．５７％，９７．９１％（ｎ＝６）；日内、日间ＲＳＤ分别为３．８３％，４．０５％，３．０９％及６．００％，４．９０％，４．７２％（ｎ＝６）。血清中药物的最低检测浓度为５ｎｇ·ｍｌ－１，在５～６００ｎｇ·ｍｌ－１血药浓度范围内线性关系良好，ｒ＝０．９９９５。结论：方法灵敏、准确、结果满意
Objective: To establish a quantitative method for the determination of itraconazole in serum samples by HPLC. Methods: The chromatographic conditions were as follows: ZORBAXTMC18 (5μm, 4.6 × 150mm) was used as the column; acetonitrilewater (67.5:32.5) as the mobile phase and the detection wavelength was 263nm; (98.5: 1.5) as extractant. Results: The recoveries of 40,80 and 300 ng · ml-1 were 105.05%, 100.57% and 97.91% respectively (n = 6). The intra-day and inter-day RSD were 3.83% 4.05%, 3.09% and 6.00%, 4.90% and 4.72% respectively (n = 6). The lowest detectable concentration of the drug in serum was 5ng · ml-1, with a good linearity in the range of 5 ~ 600ng · ml-1 plasma concentration, r = 0.9995. Conclusion: The method is sensitive, accurate and satisfactory