为了解决“五四○六”菌种的退化问题,我们从去年开始,采用冰冻低温处理,复壮“五四○六”菌种,通过反复试验,终于获得了成功。其做法是:将“五四○六”菌种放在—10℃至—30℃的低温下,经过5～7天,取出后分离培养即可。由于绝大多数孢子在低温下都丧失了活力,仅留下极少数孢于还能萌发生长成菌落,从而复壮了“五四○六”菌 In order to solve the problem of the degeneration of the “May Fourth” bacterial species, we have succeeded in using “cold and low temperature treatment” and rejuvenating the “May Fourth” species since last year. The practice is: “5460” strain on the low temperature of -10 ℃ to -30 ℃, after 5 to 7 days, remove the culture can be. Since most spores lose their vitality at low temperatures, leaving only a few spores to germinate and grow into colonies, thus rejuvenating the “May Fourth” bacteria