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肿瘤抗原可以由肿瘤细胞的MHCⅠ类分子提呈于细胞表面,经微酸法可以从肿瘤细胞表面洗脱下被MHCⅠ类分子所提呈的抗原多肽,进一步纯化富集后可获得高纯度的肿瘤抗原多既.从癌性腹水分离FBL3白血病细胞,以微酸提取液洗脱瘤细胞表面1类肽,洗脱液经Separk C18柱吸附,乙酸洗脱后,可获得分子量小于5,000D的多肽分子,冻干后即可用作肿瘤抗原多肽富集产物.骨髓诱生的树突状细胞,加人FBL3酸提抗原可有效激发FBL3特异性T细胞株的增殖,表明可以被酸提抗原多肽树突状细胞提呈给T细胞.将酸提抗原冲击后的树突状细胞回输小鼠体内,2天后,皮下接种FBL3细胞(3×10~6),而另设FBL3冻融抗原冲击DC治疗组及未处理组.酸提抗原致敏DC治疗组小鼠可诱导较
The tumor antigen can be presented on the cell surface by the MHC class I molecules of the tumor cells. The micro acid method can elute the antigen polypeptide presented by MHC I molecules from the surface of the tumor cells. After purification and enrichment, high purity tumors can be obtained. The antigen was more than one. FBL3 leukemia cells were isolated from cancerous ascites, and the peptides on the surface of tumor cells were eluted with a slightly acidic extract. The eluent was adsorbed on a Separk C18 column and eluted with acetic acid to obtain a polypeptide molecule with a molecular weight of less than 5,000D. After being lyophilized, it can be used as a tumor antigen polypeptide-enriched product. Bone marrow-derived dendritic cells, plus human FBL3 acid-extracting antigen can effectively stimulate the proliferation of FBL3-specific T cell lines, indicating that it can be acid-extracted antigen polypeptide tree The T-cells were presented to T cells. The dendritic cells impacted with the acid-extracting antigen were returned to the mice. Two days later, FBL3 cells (3×10-6) were subcutaneously inoculated, and FBL3 freeze-thawing antigen was added to impact the DCs. Treatment group and untreated group. Acid exposure antigen-sensitized DC treatment group mice can induce more