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目的探讨多ADP-核糖聚合酶[poly(ADP-ribose)polymerase,PARP]单核苷酸多态性位点Val762Ala基因多态性与急性冠状动脉综合征(acute coronary syndrome,ACS)的关系。方法选择2007年7月—2008年4月经冠状动脉造影确诊的ACS患者84例为病例组和非ACS患者87例为对照组,检测两组PARP的表达,并采用Se-quenom公司MassARRAY时间飞行质谱生物芯片系统单核苷酸多态性基因型分析技术对其进行PARP基因单核苷酸位点Val762Ala基因分型。比较两组间Val762Ala的基因型分布。结果定量分析可见病例组血浆PARP水平明显高于对照组(P<0.05)。Val762Ala位点成功分型率为98.5%,病例组与对照组TC+CC基因型频率分别为86.9%和20.7%,两组差异有统计学意义(P<0.05)。结论 PARP水平与冠状动脉粥样斑块稳定性有相关性,可能是致冠状动脉斑块破裂的重要原因之一。Val762Ala多态性与粥样硬化斑块破裂可能存在相关性。
Objective To investigate the association of Val762Ala polymorphism in ADP-ribose polymerase (PARP) single nucleotide polymorphism (SNP) with acute coronary syndrome (ACS). Methods Eighty-four ACS patients diagnosed by coronary angiography from July 2007 to April 2008 were selected as control group and 87 non-ACS patients as control group. PARP expression in both groups was detected and analyzed by Mass Spectrometry with Se-quenom time-of-flight mass spectrometry Biochip system single nucleotide polymorphism genotyping analysis PARP gene single nucleotide Val762Ala genotyping. The genotype distribution of Val762Ala between the two groups was compared. Results Quantitative analysis showed that the plasma PARP level in case group was significantly higher than that in control group (P <0.05). The successful typing rate of Val762Ala site was 98.5%. The frequencies of TC + CC genotypes in case group and control group were 86.9% and 20.7%, respectively. There was significant difference between the two groups (P <0.05). Conclusions The level of PARP is correlated with the stability of coronary atherosclerotic plaque, which may be one of the important causes of coronary artery plaque rupture. Val762Ala polymorphism and atherosclerotic plaque rupture may be related.