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目的在前期筛选出针对SEA、SEB、SEC具有广谱抑制性的多肽P72基础上,通过竞争结合实验和动物模型对多肽P72的抑制机制进行探讨。方法采用竞争结合实验检测多肽P72与MHCⅡ类分子的亲合力;利用“两次攻击(two-hit)法”建立的动物模型研究P72对SEs的体内抑制活性。结果 P72不能与FITC-SEs有效竞争结合Raji细胞上的MHCⅡ类分子,P72对SEA、SEB和SEC致Balb/c小鼠休克效应具有显著的保护作用。结论 P72可能不是与MHC II类分子结合而产生的抑制作用,P72能够在体内抑制SEs的超抗原活性,其具体的抑制机制有待深入研究。
OBJECTIVE To screen the P72 peptide with broad-spectrum inhibitory activity against SEA, SEB and SEC in the early stage and to explore the mechanism of its inhibition on P72 by competitive binding experiments and animal models. Methods The binding affinity of P72 to MHC class II molecules was detected by competitive binding assay. The inhibitory activity of P72 against SEs in vivo was studied using an animal model established by the two-hit method. Results P72 could not effectively compete with FITC-SEs for binding to MHC class II molecules on Raji cells. P72 had a significant protective effect on SEA, SEB and SEC-induced shock in Balb / c mice. Conclusion P72 may not be combined with MHC class II molecules, and P72 can inhibit the superantigen activity of SEs in vivo. The specific mechanism of inhibition needs further study.