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目的 探讨大豆异黄酮对实验性肾病综合征(NS)大鼠的治疗作用。方法 32只SD大鼠随机分为4组,A、B、c组用阿霉素(ADR)按5 mg/kg尾静脉注射复制NS大鼠模型,D组同时注射生理盐水。A组喂饲含异黄酮的大豆蛋白饲料,含量为3.4 mg大豆异黄酮/g蛋白;B组喂饲不含异黄酮的大豆蛋白;C组喂饲酪蛋白饲料;D组为正常对照。检测大鼠血生化指标(TG、Tch、TP、Alb、HDL、LDL、Ca、Fe)、肾功能指标(BUN、Cr),抗氧化功能指标(SOD、MDA、GSH、GSH-Px);并作病理光镜与电镜检查;用免疫组化法检测转化生长因子(TGF)β1的阳性率。结果 与正常组相比,A、B和C组都有高脂血症、低白蛋白血症、蛋白尿,血清TG、Tch明显升高,血清TP、Alb显著下降。同时,A组的TG、Tch及BUN显著低于C组。各组肾脏SOD、GSH差异无显著性意义;C组MDA高于A组,GSH-Px低于A组。电镜显示A、B、C组的屏障功能受损,足突细胞变性;A组病变较B、C组为轻。A组肾脏的TGF-β1阳性率低于B和C组。结论 大豆异黄酮对实验性NS大鼠肾脏功能和结构有保护作用。
Objective To investigate the therapeutic effect of soy isoflavones on experimental nephrotic syndrome (NS) rats. Methods Thirty-two Sprague-Dawley rats were randomly divided into 4 groups. Groups A, B, and c were injected with 5 mg / kg ADR into NS rat model by adriamycin (ADR), and saline was injected into group D simultaneously. Group A was fed soy protein diet containing isoflavone, the content was 3.4 mg soy isoflavone / g protein; group B fed isoflavone-free soy protein; group C fed casein feed; group D was normal control. The indexes of blood biochemistry (TG, Tch, TP, Alb, HDL, LDL, Ca, Fe), the indexes of renal function (BUN and Cr) and the indexes of antioxidation (SOD, MDA, GSH and GSH-Px) Pathological light microscopy and electron microscopy were used to detect the positive rate of transforming growth factor (TGF) β1 by immunohistochemistry. Results Compared with the normal group, hyperlipidemia, hypoalbuminemia, proteinuria, serum TG and Tch were significantly increased in groups A, B and C, and serum TP and Alb were significantly decreased. Meanwhile, TG, Tch and BUN in group A were significantly lower than those in group C. There was no significant difference in the SOD and GSH levels between groups. The MDA level in C group was higher than that in A group and GSH-Px was lower than that in A group. Electron microscope showed that barrier function of group A, B, C was impaired, dendritic cells degenerated; group A lesion was lighter than group B and C. The positive rate of TGF-β1 in group A was lower than that in group B and C. Conclusion Soy isoflavones have a protective effect on the function and structure of kidney in experimental NS rats.