论文部分内容阅读
用［３Ｈ］胸腺嘧啶核苷（［３Ｈ］ＴｄＲ）参入法，电镜，免疫组化，原位杂交方法，在自发性高血压大鼠（ＳＨＲ）观察了粉防己碱（Ｔｅｔ，０．０３μｍｏｌ·ｋｇ－１·ｄ－１×８周ｉｇ）对血管平滑肌细胞（ＶＳＭＣ）增殖的作用及对生长因子ＰＤＧＦ－Ｂ，ｂＦＧＦ的抗原表达及其相关癌基因ｃ－ｓｉｓ，ｃ－ｍｙｃｍＲＮＡ表达的影响．结果发现：Ｔｅｔ在降低ＳＨＲ血压（Ｐ＜０．０１）同时，能减少ＶＳＭＣ的线粒体，粗面内质网和［３Ｈ］ＴｄＲ参入量（Ｐ＜０．０１），并能逆转ＶＳＭＣ增殖时ＰＤＧＦ－Ｂ，ｂＦＧＦ抗原（Ｐ＜０．０５）及ｃ－ｓｉｓ，ｃ－ｍｙｃｍＲＮＡ的表达增强．提示：Ｔｅｔ抑制ＳＨＲ的ＶＳＭＣ增殖与生长因子及癌基因调控的分子生物学机制有关
The effects of tetrandrine (Tet, 0.03 μmol · L -1) were observed in spontaneously hypertensive rats (SHR) with [3H] thymidine incorporation, electron microscopy, immunohistochemistry and in situ hybridization kg-1 · d-1 × 8-week ig) on the proliferation of vascular smooth muscle cells (VSMCs) and the expression of the growth factors PDGF-B, bFGF and their related oncogene c-sis and c-mycRNA. The results showed that: Tet reduced mitochondrial, rough endoplasmic reticulum and [3H] TdR incorporation (P <0.01), and reversed the effect of PDGF -B, bFGF antigen (P <0.05) and c-sis, c-mycRNA expression increased. Tip: Tet inhibition of SHR VSMC proliferation and growth factor and oncogene regulation of the molecular biological mechanisms