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目的 :建立血清磷酸肌酸激酶同工酶 MB(CK- MB)与心肌肌钙蛋白 I(c Tn I)测定的方法 ,并通过与CK、谷草转氨酶 (AST)、乳酸脱氢酶 (L DH)比较阐述其对急性心肌梗死 (AMI)的诊断价值。方法 :共观察急性心肌梗死 (AMI) 6 5例 ,陈旧性心肌梗死 2 8例 ,稳定性心绞痛 17例 ,阵发性室上性心动过速 49例。建立 CK- MB单克隆抗体吸附测定与 c Tn I EL ISA双夹心测定的方法。同时观察血清 CK- MB、c Tn I、CK、AST、L DH在 AMI等疾患中的变化。结果 :血清 CK- MB单克隆抗体吸附法与 c Tn I EL ISA双夹心法回收率分别为 97%~ 113%、94.6 %~ 10 5 .6 % ,标准曲线相关系数分别为 0 .993与 0 .990 ,分别与琼脂糖电泳法及国外实验室检测法对比结果基本一致。 AMI胸痛发生后 12 h内血清 CK- MB的敏感性最高 ,12~ 2 4h的敏感性与 CK、c Tn I相当 ,高于 AST和L DH,而 2 4h后血清 c Tn I的敏感性最高 ;血清 CK- MB与 c Tn I对 AMI诊断的特异性和诊断有效性明显高于CK、AST、L DH。结论 :血清 CK- MB单克隆抗体吸附法与 c Tn I EL ISA双夹心法的敏感性和特异性高 ,CK- MB与c Tn I两个指标相互补充 ,对诊断 AMI有重大的临床价值
OBJECTIVE: To establish a method for the determination of serum creatine kinase MB (CK-MB) and cardiac troponin I (cTnI), and to establish a method for determination of serum cTnI by CK, aspartate aminotransferase (AST), lactate dehydrogenase ) Compared its diagnostic value of acute myocardial infarction (AMI). Methods: Sixty-five cases of AMI, 28 cases of AMI, 17 cases of stable angina pectoris and 49 cases of paroxysmal supraventricular tachycardia were observed. To establish a method for the determination of CK-MB monoclonal antibody adsorption and c Tn I EL ISA double-sandwich assay. Meanwhile, the changes of serum CK-MB, cTn I, CK, AST and L DH in AMI and other diseases were observed. Results: The recovery rates of serum CK-MB monoclonal antibody and c Tn I EL ISA double-sandwich method were 97% -113% and 94.6% -105.6% respectively, and the correlation coefficients of standard curve were 0.993 and 0 .990, respectively, agarose electrophoresis and foreign laboratory test results are basically the same. The sensitivity of serum CK-MB was the highest within 12 h after AMI, the sensitivity of 12 ~ 24 h was the same as that of CK and cTnI, but higher than that of AST and L DH, while the sensitivity of serum cTnI was the highest after 24 h The specificity and diagnostic validity of serum CK-MB and cTnI for AMI were significantly higher than those of CK, AST and L DH. Conclusion: The sensitivity and specificity of serum CK-MB monoclonal antibody adsorption method and c Tn I EL ISA double-sandwich method are high. The two indexes of CK-MB and c Tn I complement each other and have great clinical value in the diagnosis of AMI