溶胶凝胶过程以其纯度高,均匀性强,处理温度低,反应条件易于控制等优点成为生物传感器中一种颇具前途的固化方法.利用硅酸乙酯(TEOS)的溶胶凝胶化过程对葡萄糖氧化酶(GOD)进行固化,并制备了不同载体下的GOD酶柱.实验结果表明GOD可在SiO_2的溶胶凝胶体中保持较高的活性.将此酶柱用于流动注射化学发光分析体系,可实现对葡萄糖的在线测定.该体系的校正曲线线性范围为3.5～70μmol/L,其相关系数为0.999,检出下限为0.6μmol/L. The sol-gel process has become a promising solidification method in biosensors because of its high purity, high homogeneity, low processing temperature and easy control of reaction conditions. Sol-gel process using TEOS Glucose oxidase (GOD) were immobilized and GOD enzyme columns were prepared under different carriers.The experimental results showed that GOD could maintain a high activity in sol-gel of SiO_2.The enzyme column was used for flow injection chemiluminescence System, the on-line determination of glucose can be achieved.The calibration curve of this system has a linear range of 3.5 ~ 70μmol / L with a correlation coefficient of 0.999 and a detection limit of 0.6μmol / L.