[目的]应用SDS—AGE非浓缩尿蛋白电泳技术,分析糖尿病肾病患者蛋白尿成分特点。[方法]采用全自动琼脂糖凝胶电泳分析仪对20例正常对照组及40例糖尿病患者进行尿蛋白电泳分析。[结果]40例糖尿病患者尿蛋白经SDS-AGE电泳后。根据蛋白质分子量的大小顺序排列在凝胶上可区分为肾小球型、肾小管型、混合型、溢出型及生理型蛋白尿。[结论]非浓缩尿蛋白电泳分析,能早期敏感的检测尿蛋白组成成分的变化,当医院条件缺乏肾组织活检时,可为临床医生提供诊断糖尿病肾病,肾脏损伤部位和程度的依据。 [Objective] To analyze the characteristics of proteinuria in patients with diabetic nephropathy by SDS-AGE non-concentrated urine protein electrophoresis. [Method] Urine protein electrophoresis analysis was performed on 20 normal controls and 40 cases of diabetic patients by using an automatic agarose gel electrophoresis analyzer. [Results] Urine protein in 40 cases of diabetic patients after SDS-AGE electrophoresis. According to the order of the molecular weight of the protein in the gel can be divided into glomerular type, tubule type, mixed type, overflow type and physiological proteinuria. [Conclusion] Non-concentrated urinary protein electrophoresis analysis can detect the changes of urinary protein components sensitively at early stage. When hospital conditions lack the renal biopsy, it can provide clinicians with basis for diagnosis of diabetic nephropathy and kidney injury site and extent.