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以草炭、蛭石、珍珠岩混合物料为基质,并添加壳聚糖,进行番茄穴盘育苗试验,测定幼苗生长发育参数,并采用454焦磷酸测序技术分析穴盘苗根际细菌的种群变化。结果表明:添加壳聚糖对基质pH值无显著影响,但显著降低了生长后期基质EC值。番茄穴盘苗根际细菌优势菌群包括丛毛单胞菌科食酸菌属(8.92%)、丰祐菌科丰佑菌属(5.03%)以及草酸杆菌科Massilia属(2.43%)等。基质添加壳聚糖显著促进番茄穴盘苗的生长。壳聚糖引起根际细菌群落的丰富度和多样性降低,优势细菌主要包括丛毛单胞菌科Pelomonas属(13.52%)、假单胞菌科纤维弧菌属(8.21%)、根瘤菌科根瘤菌属(2.91%)和亚硝化单胞菌科亚硝化螺菌属(1.04%)等,这些优势细菌多具有植物促生潜力。添加壳聚糖对根际细菌具有定向调控作用,可以显著改善番茄穴盘苗根际基质的细菌群落结构。
The mixture of peat, vermiculite and perlite was used as substrate, and chitosan was added to test the growth of tomato seedlings. The 454 pyrophosphate sequencing technique was used to analyze the population changes of bacteria in the rhizosphere of plug seedlings. The results showed that adding chitosan had no significant effect on the pH value of the matrix, but significantly reduced the matrix EC value at the late growth phase. The dominant flora of tomato plug seedling rhizosphere bacteria include Comamonas group (8.92%), Fengyunfeng affiliates (5.03%) and oxalicobacteriaceae Massilia (2.43%). Substrate addition of chitosan significantly promoted the growth of tomato plug seedlings. Chitosan caused a decrease in the abundance and diversity of the rhizosphere bacteria community. The predominant bacteria included Pelothia species (13.52%), Pseudomonas spp. (8.21%), Rhizobiaceae Rhizobium (2.91%) and Nitrosomonas nitrosospirillum (1.04%), etc. These predominant bacteria have more plant growth potential. Chitosan added to the rhizosphere bacteria directional regulation, can significantly improve the tomato plug seedling rhizosphere bacterial community structure.