【摘 要】
:
AIM: To investigate the sphingosine 1-phosphate (S1P) receptor expression profile in human esophageal cancer cells and the effects of S1P5 on proliferation and
【机 构】
:
Department of Microbiology and Immunology,Institute of Immunology and Molecular Biology,Department o
【基金项目】
:
The Key Project of Ministry of Education;Sichuan Youth Science and Technology Foundation;Key Laboratory Foundation of North Sichuan Medical
论文部分内容阅读
AIM: To investigate the sphingosine 1-phosphate (S1P) receptor expression profile in human esophageal cancer cells and the effects of S1P5 on proliferation and migration of human esophageal cancer cells. METHODS: S1P receptor expression profile in human esophageal squamous cell carcinoma cell line Eca109 was detected by semiquantitative reverse transcription polymerase chain reaction. Eca109 cells were stably transfected with S1P5EGFP or controlEGFP constructs. The relation between the responses of cell proliferation and migration to S1P and S1P5 expression was evaluated by 3(4,5dimethylthiazol2yl) -2,5diphenyl tetrazolium bromide and migration assay, respectively. RESULTS: Both normal human esophageal mucosal epithelium and Eca109 cells expressed S1P1, S1P2, S1P3 and S1P5, respectively. Esophageal mucosal epithelium expressed S1P5 at a higher level than Eca109 cell line. S1P5 overexpressing Eca109 cells displayed spindle cell morphology with elongated and extended filopodialike projections. The proliferation response of S1P5transfec ted Eca109 cells was lower than that of control vectortransfected cells with or without S1P stimulation ( P < 0.05 or 0.01). S1P significantly inhibited the migration of S1P5transfected Eca109 cells ( P < 0.001). However, without S1P in transwell lower chamber, the number of migrated S1P5transfected Eca109 cells was greater than that of control vectortransfected Eca109 cells ( P < 0.001). CONCLUSION: S1P binding to S1P5 inhibits the proliferation and migration of S1P5transfected Eca109 cells. Esophageal cancer cells may downregulate the expression of S1P5 to escape the inhibitory effect.
其他文献
保护层开采及被保护层卸压瓦斯抽采技术是防治煤与瓦斯突出最有效、最经济的区域性措施之一.通过分析保护层开采的机理和保护效果的影响因素,在反映保护层厚度与层间垂距的相
Background The definitive treatment for myocardial ischemia is reperfusion.However,reperfusion injury has the potential to cause additional reversible and irrev
In this work,by a combination of controlled ring-opening polymerization(CROP)and click reaction,we reported a facile and useful method to synthesize linear poly
细根对植物群落功能的发挥和土壤碳库及全球碳循环具有重要意义.采用土柱法和分解袋法,于2010年5-10月整个生长季内,对三工河流域梭梭(Haloxylon ammodendron)群落的细根生物
该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生、测量监控等方面人手,介绍了S226海滨大桥
Soils play a critical role in the global carbon cycle,and can be major source or sink of CO2 depending upon land use,vegetation type and soil management practic
The problem of hydrate blockage of pipelines in offshore production is becoming ever-increasing severe because oil fields in ever-increasing unusual environment
The investigation of dissolved organic matter (DOM) on urease, catalase and polyphenol oxidase activity in a phenanthrene (Phe)-contaminated soil was conducted
This paper concerns the mission scheduling problem for an agile Earth-observing satellite. Mission planning and action planning for the satellite are both taking into account. Multiple mission types(
Background The persistence of sleep disordered breathing (SDB) symptoms after tonsil and/or adenoid (T&A) surgery are common in children with obstructive sleep