目的:探讨n-3多不饱和脂肪酸(n-3 PUFA)对早期非酒精性脂肪肝(NAFLD)的治疗作用,并通过靶向代谢组学测定n-3PUFA代谢谱,以期寻找有效的代谢产物并研究其机制。方法:将C57BL/6雄性小鼠分为3组,分别给予正常饮食(ND)、45%的高脂饮食(HFD)和添加3%n-3 PUFA的高脂饮食(HFD+n-3 PUFA)喂养4 d。结果:短期HFD增加了小鼠肝脏的甘油三 Objective: To investigate the therapeutic effect of n-3 polyunsaturated fatty acid (n-3 PUFA) on early non-alcoholic fatty liver disease (NAFLD) and to determine n-3PUFA metabolite profile by targeted metabolomics in order to find effective metabolites And study its mechanism. Methods: C57BL / 6 male mice were divided into three groups and were given normal diet (ND), 45% high fat diet (HFD) and 3% n-3 PUFA high fat diet (HFD + n-3 PUFA ) For 4 days. Results: Short-term HFD increased glycerol in mouse liver