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目的分析导致儿童咽扁桃体炎的酿脓链球菌(Streptococcus pyogenes),即A族β溶血性链球菌(group A streptococcus,GAS)的emm分型。方法 2007年1—12月共获取119株GAS,分别来自于重庆(42株)、深圳(30株)和上海(47株)3个地区。应用多聚合酶链反应(PCR)扩增编码成熟M蛋白基因(emm基因)。对PCR扩增后的emm基因产物进行测序,并将所测得的emm基因序列提交到相关网站,获取emm分型结果。结果共有13种emm型GAS可导致儿童发生咽扁桃体炎:emm1.0(24.37%),emm3.1(0.83%),emm4.0(0.83%),emm6.5(1.68%),emm12.0(55.46%),emm22.0(10.08%),emm63.0(0.83%),emm77.0(0.83%),emm80.0(0.83%),emm102.2(1.68%),emm104.0(0.83%),st1815.0(0.83%)和stG485.0(0.83%)。重庆地区可见10种emm分型:emm1.0(11.90%),emm3.1(2.38%),emm6.5(4.76%),emm12.0(61.90%),emm22.0(4.76%),emm63.0(2.38%),emm80.0(2.38%),emm102.2(4.76%),st1815.0(2.38%)和stG485.0(2.38%)。深圳地区可见5种emm分型:emm1.0(16.67%),emm12.0(50.00%),emm22.0(26.67%),emm77.0(3.33%)和emm104.0(3.33%)。上海地区可见4种emm分型:emm1.0(40.42%),emm4.0(2.13%),emm12.0(53.19%)和emm22.0(4.26%)。结论 emm1.0、emm12.0和emm22.0克隆流行菌株是最常见的导致重庆、深圳和上海3地区儿童发生咽扁桃体炎的酿脓链球菌。3地区导致儿童发生咽扁桃体炎的克隆流行菌株存在差别。
Objective To analyze the emm typing of Streptococcus pyogenes, group A streptococcus (GAS), which causes pharyngotonsitis in children. Methods A total of 119 GAS were obtained from January 2007 to December 2007 in Chongqing (42 strains), Shenzhen (30 strains) and Shanghai (47 strains). Polymerase chain reaction (PCR) amplification of the mature M protein gene (emm gene) was amplified. The emm gene product after PCR amplification was sequenced, and the emm gene sequence was submitted to relevant websites for emm typing results. Results A total of 13 emm GASs resulted in pharyngitis in children: emm1.0 (24.37%), emm3.1 (0.83%), emm4.0 (0.83%), emm6.5 (55.46%), emm22.0 (10.08%), emm63.0 (0.83%), emm77.0 (0.83%), emm80.0 (0.83%), emm102.2 (1.68%), emm104.0 %), st1815.0 (0.83%) and stG485.0 (0.83%). There were 10 emm types in Chongqing: emm1.0 (11.90%), emm3.1 (2.38%), emm6.5 (4.76%), emm12.0 (61.90%), emm22.0 (4.76%), emm63 .0 (2.38%), emm80.0 (2.38%), emm102.2 (4.76%), st1815.0 (2.38%) and stG485.0 (2.38%). There are five emm types in Shenzhen: emm1.0 (16.67%), emm12.0 (50.00%), emm22.0 (26.67%), emm77.0 (3.33%) and emm104.0 (3.33%). There are four emm types in Shanghai: emm1.0 (40.42%), emm4.0 (2.13%), emm12.0 (53.19%) and emm22.0 (4.26%). Conclusions The most common clonal strains of emm1.0, emm12.0 and emm22.0 are Streptococcus pyogenes which cause pharyngotonsillitis in children in Chongqing, Shenzhen and Shanghai. There are differences between the clonogenic strains that cause pharyngotonsillaritis in children in the three regions.