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目的:本实验探讨组蛋白去乙酰化酶抑制剂PXD101对口腔鳞癌HN-6细胞的增殖、细胞凋亡及细胞周期的影响。方法:PXD101对口腔鳞癌HN-6细胞进行干预,倒置相差显微镜观察细胞形态学改变;MTT法检测PXD101对HN-6细胞的增殖影响;Annexin-V-FITC/PI双染流式细胞仪定量检测细胞凋亡;流式细胞仪分析细胞周期。结果:PXD101可明显抑制HN-6细胞的生长(P<0.05),呈时间剂量依赖性。倒置相差显微镜下观察对照组细胞贴壁,形态呈多边形,生长活跃;实验组细胞脱壁,变小,细胞核皱缩。绘制细胞生长曲线示,随着PXD101的浓度和作用时间的增加,HN-6细胞生长明显受到抑制,各实验组细胞生长抑制率与对照组比较,P<0.05差别有统计学意义。1μmol/LPXD101作用24 h,48 h细胞总凋亡率分别为20.9%、38.6%,与对照组相比有统计学意义(P<0.05);HN-6细胞周期阻滞于G0/G1期,与对照组相比,P<0.05差别有统计学意义。结论:PXD101体外实验能显著抑制人口腔舌癌HN-6的细胞增殖,诱导细胞凋亡。
AIM: To investigate the effects of histone deacetylase inhibitor PXD101 on proliferation, apoptosis and cell cycle of oral squamous cell carcinoma HN-6 cells. Methods: PXD101 was used to infect HN-6 cells in oral squamous cell carcinoma, morphological changes of cells were observed by inverted phase contrast microscope. The proliferation of HN-6 cells was detected by MTT assay and Annexin-V-FITC / PI double staining flow cytometry Apoptosis was detected; cell cycle was analyzed by flow cytometry. Results: PXD101 could significantly inhibit the growth of HN-6 cells (P <0.05) in a dose-and time-dependent manner. Under inverted phase contrast microscope, the cells in the control group adhered to the wall, the morphology was polygonal, and the growth was active. In the experimental group, the cells detached, became smaller and the nucleus was shrunk. The growth curves of HN-6 cells were significantly inhibited as the concentration of PXD101 and the action time increased. The inhibition rate of cell growth in each experimental group was significantly different from that of the control group (P <0.05). The apoptosis rate of HN-6 cells at 24 h and 48 h after treatment with 1 μmol / L LPD101 was 20.9% and 38.6%, respectively, which was significantly higher than that of the control group (P <0.05) Compared with the control group, P <0.05 difference was statistically significant. Conclusion: PXD101 can significantly inhibit cell proliferation and induce apoptosis of oral tongue cancer HN-6 cells in vitro.