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目的研究心脑肾康对自由基的清除作用。方法连续给大鼠灌胃心脑肾康7 d后,以线栓法建立大脑中动脉梗死(MCAO)缺血再灌注模型,缺血1.5 h再灌注24 h后断头取脑,检测大脑皮层丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)、活性氧(ROS)、谷胱甘肽(GSH)含量。结果与模型组相比,高、中、低3个剂量心脑肾康用药后均能显著降低缺血后脑组织匀浆中MDA、NO、ROS含量,增加SOD、GSH活性(P<0.05)并呈剂量依赖性。其中,高剂量效果最好,优于阳性对照药(P<0.05)。结论心脑肾康具有良好的自由基清除作用。
Objective To study the effects of Xin Naoshenkang on free radical scavenging. METHODS: Rats were treated with continuous gavage for 7 days. The middle cerebral artery occlusion (MCAO) ischemia-reperfusion model was established by thread embolism method. The brain was decapitated after ischemia for 1.5 h and reperfusion for 24 h, and the cerebral cortex was examined. Malondialdehyde (MDA), superoxide dismutase (SOD), nitric oxide (NO), reactive oxygen species (ROS), glutathione (GSH) content. Results Compared with the model group, the three doses of high-, middle-, and low-dose XinNaoshenkang significantly decreased the content of MDA, NO, ROS in the brain homogenate after ischemia, and increased the activities of SOD and GSH (P<0.05). In a dose-dependent manner. Among them, the high dose effect was the best, better than the positive control drug (P<0.05). Conclusion Xinnaoshenkang has good free radical scavenging effect.