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花生野生种Arachiscardenasii与栽培品种以常规方法杂交，其杂交成功率和杂种生活力均较低。利用胚营救技术将杂交获得的未成熟胚珠和胚进行离体培养，可诱导幼胚分化发育或使胚成熟萌发。离体培养杂种小苗的带腋芽茎段，用MS＋BA4ms／L＋CH600mg／L培养基诱导产生了愈伤组织和芽原基。通过继代增殖培养，平均每个愈伤组织获得22．6和24．6个芽原基以及6．1和7．2个无根小苗。经诱根培养（MS＋NAA0．2mg／L），再生苗即生长成为完整植株。
Arachiscardenasii, a peanut wild species, was crossed with cultivars by conventional methods, and its success rate of hybridization and hybrid vigor were low. Using embryo rescue technology, the immature ovules and embryos obtained from hybridization can be cultured in vitro to induce immature embryo differentiation or germination. The hybrid shoots with axillary buds were cultured in vitro, and callus and bud primordia were induced by MS + BA4ms / L + CH600mg / L medium. By subculture, 22.6 and 24.6 bud primordia and 6.1 and 7.2 rootless seedlings were obtained on average for each callus. Induced by rooting (MS + NAA0.2mg / L), the regeneration of seedlings that grow into a complete plant.