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BACKGROUND:Calretinin and parvalbumin are members of the intracellular calcium binding protein family,which transform Ca2+ bioinformation into regulation of neuronal and neural network activities.OBJECTIVE:To observe expression and co-expression of calretinin and parvalbumin in rat facial nucleus neurons.DESIGN,TIME AND SETTING:Neuronal morphology experiment was performed at the Research Laboratory of Applied Anatomy,Department Neurobiology and Anatomy,Xiangya Medical College of Central South University from August to October 2007.MATERIALS:Five healthy,adult Sprague Dawley rats were selected.Polyclonal rabbit-anti-parvalbumin and mouse-anti-calretinin were provided by Sigma,USA.METHODS:Rat brains were obtained and cut into coronal slices using a freezing microtome.Slices from the experimental group were immunofluorescent stained with polyclonal rabbit-anti-parvalbumin and mouse-anti-calretinin antibodies.The control group sections were stained with normal rabbit and mouse sera.MAIN OUTCOME MEASURES:Immunofluorescent double-staining was used to detect calretinin and parvalbumin expression.Nissl staining was utilized for facial nucleus localization and neuronal morphology analysis.RESULTS:The majority of facial motor neurons was polygon-shaped,and expressed calretinin and parvalbumin.The calretinin-immunopositive neurons also exhibited parvalbumin immunoreactivity,that is,calretinin and parvalbumin were co-expressed in the same neuron.CONCLUSION:Calretinin and parvalbumin were expressed in facial nucleus neurons,with varied distribution.
BACKGROUND: Calretinin and parvalbumin are members of the intracellular calcium binding protein family, which transform Ca2 + bioinformation into regulation of neuronal and neural network activities. OBJECTIVE: To observe expression and co-expression of calretinin and parvalbumin in rat facial nucleus neurons. DESIGN, TIME AND SETTING: Neuronal morphology experiment was performed at the Research Laboratory of Applied Anatomy, Department Neurobiology and Anatomy, Xiangya Medical College of Central South University from August to October 2007. MIALIALS: Five healthy, adult Sprague Dawley rats were selected. Polyclonal rabbit-anti -parvalbumin and mouse-anti-calretinin were provided by Sigma, USA. METHODS: Rat brains were obtained and cut into coronal slices using a freezing microtome .lices from the experimental group were immunofluorescent stained with polyclonal rabbit-anti-parvalbumin and mouse-anti -calretinin antibodies. The control group sections were stained with normal rabbit and mouse sera. MAIN OU TCOME MEASURES: Immunofluorescent double-staining was used to detect calretinin and parvalbumin expression. Nissl staining was utilized for facial nucleus localization and neuronal morphology analysis .RESULTS: The majority of facial motor neurons was polygon-shaped, and expressed calretinin and parvalbumin. Calretinin -immunopositive neurons also showed parvalbumin immunoreactivity, that is, calretinin and parvalbumin were co-expressed in the same neuron. CONCLUSION: Calretinin and parvalbumin were expressed in facial nucleus neurons, with varied distribution.