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目的:研究泻火补肾汤对神经干细胞(NSCs)移植入脑出血大鼠脑室下区(SVZ)后转化生长因子(TGF-β1)和血管细胞间黏附分子(VCAM-1)表达的影响,探讨泻火补肾汤能否诱导移植免疫耐受。方法:将SD大鼠随机分为6组:A(正常组)5只,B(假手术组)、C(模型组)、D(模型治疗组)、E(移植组)、F(移植治疗组)各5只。采用免疫组织化学方法观察术后2 d4、d、7 d、14 d、28 d TGF-β1和VCAM-1的表达。结果:B、C、D、E、F五组VCAM-1和TGF-β1分别在2 d4、d表达达高峰(P<0.05)。VCAM-1在各时间点E组表达最强(P<0.05),而TGF-β1在F组表达最强(P<0.01)。同时间点除28 d外,F组比E组、D组比C组TGF-β1表达增强(P<0.05),而VCAM-1表达减弱(P<0.05)。结论:泻火补肾汤可通过促进免疫抑制因子TGF-β1的表达,抑制免疫相关因子VCAM-1的表达,从而有助于移植受体免疫耐受。
Objective: To study the effect of Xiexie Bushen decoction on the expression of transforming growth factor (TGF-β1) and vascular cell adhesion molecule (VCAM-1) after neural stem cells (NSCs) transplantation into the subventricular zone (SVZ) of rats with intracerebral hemorrhage. Does Xiehuo Bushen Decoction Induce Transplantation Immune Tolerance? Methods: SD rats were randomly divided into 6 groups: A (normal group) 5, B (sham group), C (model group), D (model treatment group), E (transplant group), F (transplantation therapy) Group) 5 each. Immunohistochemistry was used to observe the expression of TGF-β1 and VCAM-1 at 2 d4, d, 7 d, 14 d, and 28 d after operation. Results: The expressions of VCAM-1 and TGF-β1 in B, C, D, E and F groups reached the peak on the 2nd and 4th day respectively (P<0.05). VCAM-1 expressed most strongly in group E at each time point (P<0.05), while TGF-β1 was most strongly expressed in group F (P<0.01). At the same time, except for 28 days, the expression of TGF-β1 in group F and group D was higher than that in group C (P<0.05), while the expression of VCAM-1 was decreased (P<0.05). Conclusion: Xiehuo Bushen Decoction can promote the immune tolerance of transplant recipient by promoting the expression of immunosuppressive factor TGF-β1 and inhibiting the expression of immune-related factor VCAM-1.