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目的:探讨急性冠状动脉综合征(ACS)患者胆固醇酯转运蛋白(CETP)TaqIB基因多态性与血脂水平的关系。方法:对236例经冠状动脉造影证实为ACS的患者(ACS组)及54例经冠状动脉造影排除冠心病的患者(对照组)进行研究。采用酶法测定血脂各项水平,采用多聚酶链反应-限制性内切酶片段长度多态性分析CETP基因中TaqIB基因多态性。结果:与对照组比较,ACS组CETPTaqIB基因型及等位基因频率的分布差异无统计学意义(P>0.05)。B1B2与B1B1基因型比较,HDL-C水平显著增高(P<0.05)。B1B2与B2B2基因型比较各血脂指标间差异无统计学意义。等位基因B2与低HDL-C有关。TG≥1.7mmol/L时不同基因型间各血脂指标差异无统计学意义;但在TG<1.7mmol/L时B1B1基因型的HDL-C水平显著低于B1B2基因型(P<0.05)。结论:B1、B2等位基因频率的分布在ACS组与对照组间差异无统计学意义。TG及TaqIB基因多态性均可影响HDL-C水平。
Objective: To investigate the relationship between cholesterol ester transporter (CETP) TaqIB gene polymorphism and serum lipids in patients with acute coronary syndrome (ACS). Methods: A total of 236 patients with ACS confirmed by coronary angiography (ACS group) and 54 patients with coronary artery disease excluded from coronary angiography (control group) were studied. The levels of lipids were determined by enzymatic method. The polymorphism of TaqIB gene in CETP gene was analyzed by polymerase chain reaction-restriction fragment length polymorphism. Results: Compared with the control group, there was no significant difference in the distribution of CETPTaqIB genotype and allele frequency in ACS group (P> 0.05). The levels of HDL-C in B1B2 and B1B1 genotypes were significantly higher (P <0.05). There was no significant difference between B1B2 and B2B2 genotypes in each lipid index. Allele B2 is associated with low HDL-C. There was no significant difference in serum lipids between different genotypes when TG≥1.7mmol / L; however, HDL-C level of B1B1 genotype was significantly lower than that of B1B2 genotype (P <0.05) at TG <1.7mmol / L. Conclusion: There is no significant difference in the frequency distribution of B1 and B2 alleles between ACS group and control group. TG and TaqIB gene polymorphisms can affect HDL-C levels.