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目的探讨骨桥蛋白(OPN)对小鼠体外受精及早期胚胎发育的影响。方法 120只昆明雌鼠和30只雄鼠,采用体外受精、胚胎培养方法,将小鼠精子、卵子、原核期胚胎和2-细胞期胚胎分别用不同浓度的OPN抗体预处理,观察小鼠受精、卵裂以及早期胚胎发育情况。结果不同浓度的OPN抗体分别预处理精子和卵子后,与对照组比较,受精率显著降低(P<0.01)。OPN抗体预处理原核期胚胎后,0.01mg/L OPN抗体组的卵裂率较对照组低,但差异无显著性(P=0.052),1.00mg/L OPN抗体组的卵裂率低于0.01mg/L组(P<0.01)及0.10mg/L组(P<0.05)。不同浓度的OPN抗体预处理2-细胞胚胎后可抑制胚胎发育。1.00mg/L OPN抗体组的4-细胞率和8-细胞率与0.10mg/L OPN抗体组相比差异无显著性(P>0.05),但前者的囊胚形成率显著低于后者(P<0.01)。1.00mg/L OPN抗体组的4-细胞率、8-细胞率以及囊胚形成率均显著低于0.01mg/L OPN抗体组(P<0.01)。结论 OPN可促进小鼠的受精和早期胚胎发育。
Objective To investigate the effect of osteopontin (OPN) on in vitro fertilization and early embryo development in mice. Methods 120 Kunming female rats and 30 male rats were fertilized by in vitro fertilization and embryo culture. The spermatozoa, eggs, prokaryotic embryos and 2-cell embryos were pretreated with different concentrations of OPN antibody respectively. The fertilization, Cleavage and early embryonic development. Results Different doses of OPN antibody pretreatment of sperm and egg, compared with the control group, fertilization rate was significantly reduced (P <0.01). OPN antibody pretreatment prokaryotic embryos, OPN antibody group 0.01mg / L cleavage rate lower than the control group, but no significant difference (P = 0.052), 1.00mg / L OPN antibody cleavage rate of less than 0.01 mg / L group (P <0.01) and 0.10 mg / L group (P <0.05). Different concentrations of OPN antibody pretreatment of 2-cell embryos can inhibit embryonic development. There was no significant difference in the 4-cell ratio and 8-cell ratio between the OPN antibody group and the OPN antibody group (P> 0.05) at 1.00mg / L OPN antibody group, but the blastocyst formation rate of the former group was significantly lower than the latter P <0.01). The 4-cell rate, 8-cell rate and blastocyst formation rate of OPN antibody group were significantly lower than that of 0.01mg / L OPN antibody group (P <0.01). Conclusion OPN can promote mouse fertilization and early embryo development.