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作者用减毒沙门菌作为编码白细胞介素4 ( IL - 4)和 IL - 1 8的原核表达质粒传递系统 ,并评估其在不同实验模型中调节免疫应答的能力。 作者首先将鼠 IL- 4和 IL- 1 8基因的编码序列克隆于由巨细胞病毒 ( CMV)启动子控制的 VR1 0 1 2质粒 ,然后将这些构建体电穿孔入伤寒杆菌
The authors used attenuated Salmonella as a prokaryotic expression plasmid delivery system encoding interleukin - 4 (IL - 4) and IL - 18 and evaluated its ability to modulate immune responses in different experimental models. The authors first cloned the coding sequences for the murine IL-4 and IL-18 genes into the VR1 012 plasmid controlled by the cytomegalovirus (CMV) promoter and then electroporated these constructs into S. typhi