论文部分内容阅读
目的:比较不同时间经动脉溶栓治疗对血栓栓塞性脑缺血大鼠损伤的保护作用及大黄苷元对脑缺血溶栓治疗时间窗的影响。方法:大鼠随机分组。制备血栓栓塞性脑缺血动物模型。缺血后大鼠3、6、9h经导管区域动脉溶栓。动脉给药后24h,观察大鼠脑组织病理损伤及神经症状,测定脑组织含水量和脑梗塞面积变化,观察颅内出血率。结果:各模型组大鼠均较假手术组神经症状评分增高,脑组织含水量增加,脑梗塞面积增大,脑组织病理损伤明显;溶栓组和大黄苷元组各时间点大鼠神经症状评分降低、脑组织含水量减少、脑梗塞面积减小、脑病理损伤减轻;各用药3h组上述指标的改善均较9h组明显;溶栓组颅内出血率较模型组和大黄苷元组增高,联合组较溶栓组降低。结论:脑组织受损随缺血时间延长而加重;不同时间窗经动脉溶栓对脑缺血损伤均具有保护作用,但缺血后3h和6h溶栓效果明显优于9h;大黄苷元可增强溶栓效果,具有延长溶栓时间窗、降低溶栓后颅内出血率的作用。
Objective: To compare the protective effect of arterial thrombolysis on the injury of thromboembolic cerebral ischemia rats and the influence of rhubarb aglycone on the time window of thrombolytic therapy. Methods: Rats were randomly divided into groups. Preparation of thromboembolic cerebral ischemia in animal models. Rats were subjected to thrombolysis via catheter at 3, 6 and 9 hours after ischemia. 24h after arterial administration, the pathological damage and neurological symptoms of rat brain were observed. The changes of brain water content and infarct size were observed and the rate of intracranial hemorrhage was observed. Results: Compared with sham operation group, neurological symptom score increased, brain water content increased, infarct size increased, and pathological damage of brain tissue was obvious in each model group. Neurological symptoms of rats in thrombolysis group and rhubarb aglycone group Reduce the brain water content, reduce the area of cerebral infarction, alleviate the pathological damage of brain; the improvement of the above indexes in 3h groups of each medication was more obvious than 9h group; the intracranial hemorrhage rate of thrombolytic group was higher than the model group and the rhubarb aglycone group, The combined group was lower than the thrombolytic group. Conclusion: The damage of brain tissue aggravates with the prolongation of ischemic time. Thrombolysis with arterial thrombolysis at different time windows has a protective effect on cerebral ischemic injury, but the thrombolytic effect at 3h and 6h after ischemia is significantly better than that of 9h. Enhance the thrombolytic effect, with the extension of thrombolysis time window, reduce the rate of intracranial hemorrhage after thrombolysis.