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目的研究宫颈癌患者中微卫星基因多态性,分析微卫星基因与宫颈癌的发病及病变程度的相关性。方法研究对象选择宫颈癌和癌前病变(CIN)患者,应用DNA遗传分析仪检测,既能稳定遗传又具有多态性的遗传标志物微卫星DNA(即STR),分析微卫星DNA多态性与疾病的关系。结果检出45名CIN和92名宫颈癌患者的微卫星DNA遗传多态性,并发现CIN组有3个等位基因与对照组有统计学差异,其中等位基因D21S11*32.2的频率(0.167)>对照组(0.075),χ2=5.623,P<0.05。等位基因D19S433*15.2、D2S1338*24频率分别是为0.111、0.078,均<对照组的0.225、0.18,χ2分别为5.238,5.136,P值分别为0.023、0.031。
Objective To study the polymorphism of microsatellite DNA in patients with cervical cancer and to analyze the correlation between microsatellite genes and the incidence of cervical cancer. Methods Subjects selected cervical cancer and precancerous lesions (CIN) patients, the application of DNA genetic analyzer detection, both stable genetic polymorphic genetic markers microsatellite DNA (STR), analysis of microsatellite DNA polymorphisms The relationship with the disease. Results The genetic polymorphisms of microsatellite DNA in 45 patients with CIN and 92 cervical cancers were detected. There were statistically significant differences among the three alleles in CIN group and control group. The frequency of allele D21S11 * 32.2 (0.167 )> Control group (0.075), χ2 = 5.623, P <0.05. The frequencies of alleles D19S433 * 15.2 and D2S1338 * 24 were 0.111,0.078 respectively, both <0.225,0.18 and χ2 values of 5.238 and 5.136, respectively, with P values of 0.023 and 0.031, respectively.