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目的 探讨外源性血小板源伤口愈合因子 (platelet derivedwoundhealingfactors,PDWHF)改善糖尿病难愈性伤口愈合的作用与 β -转化生长因子 (TGF - β)基因表达的关系。 方法 44只雄性SD大鼠 ,分为正常对照组 (鼠数 =12 )、糖尿病组 (鼠数 =32 )。四氧嘧啶诱导糖尿病组大鼠血糖值 >1.8g/L 1～ 2d后 ,在每组大鼠背部用外科方法剪去 2块直径为 1.8cm的全层皮肤 ,造成全皮层开放伤口。术后当日及以后连续 6日 ,选糖尿病大鼠一侧伤口为PDWHF治疗组伤口 ,在其局部应用PDWHF ( 10 0 μg/伤口 ) ,另一侧 (糖尿病对照组伤口 )及正常对照组伤口不予PDWHF治疗。 结果 伤后 5d ,PDWHF治疗组伤口与糠尿病对照组伤口闭合指数无差异 ,均低于正常对照组 (P <0 .0 5 ) ;伤后 7,10 ,14d ,PDWHF治疗组伤口闭合程度明显高于糠尿病对照组伤口(P <0 .0 5 ) ,但仍低于正常对照组 (P <0 .0 5 )。伤后 5 ,7d ,PDWHF治疗组伤口组织中TGF - βmRNA水平量是糖尿病对照组的 4倍和 5 .6倍 (P <0 .0 1) ,但低于正常对照组 (P <0 .0 5 ) ;伤后 10d ,3组间TGF - βmRNA水平量无明显差异。 结论 PDWHF促糖尿病大鼠伤口愈合与其增强伤口组织TGF - β基因表达相关。
Objective To investigate the relationship between the effect of exogenous platelet derived wound healing factor (PDWHF) on the healing of diabetic refractory wound and the expression of β - transforming growth factor (TGF - β) gene. Methods Forty-four male SD rats were divided into normal control group (the number of mice = 12) and diabetic group (the number of rats = 32). Alloxan-induced diabetes rats blood glucose> 1.8g / L 1 ~ 2d, the back of each rat was surgically cut 2 full-thickness skin 1.8cm in diameter, resulting in a cortical open wound. The wounds on the side of diabetic rats were treated with PDWHF (10 0 μg / wound) on the 6th day after surgery and on the other side (the wounds of the diabetic control group) and the normal control group To PDWHF treatment. Results There was no significant difference in wound closure index between the PDWHF treatment group and the control group (P <0.05), and the PDWHF treatment group was significantly lower than the normal control group (P <0.05) Which was significantly higher than that of the control group (P <0.05), but still lower than that of the normal control group (P <0.05). On the 5th and 7th day after injury, the levels of TGF - βmRNA in the wounds of PDWHF group were 4 times and 5.6 times (P <0.01) higher than those in the normal control group (P <0. 0) 5). There was no significant difference in the level of TGF - βmRNA between the three groups 10d after injury. Conclusion PDWHF induces wound healing in diabetic rats and enhances the expression of TGF - β gene in wound tissues.