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个体对IDDM的易感性与人类主要组织相容抗原复合体——HLA的D位点相关。在不同人群中与IDDM易感相关的基因也不尽相同。关于中国人IDDM的易感基因了解基少。本文应用聚合酶链反应(PCR)技术结合斑点杂交和特定位点酶切分析的方法,检测45例非IDD M和10例IDDM的HLA-DQA_1,HLA-DQB_1基因第二外显子结构。10例IDDM均于20岁以前发病,病程3年以上,都有酮症酸中毒。对照者为同龄的志愿献血者。
The individual’s susceptibility to IDDM correlates with the D site of HLA, a major histocompatibility antigen complex. The genes associated with IDDM susceptibility in different populations are also different. About Chinese IDDM gene susceptibility to understand less. In this study, polymerase chain reaction (PCR) technology combined with dot blot hybridization and site-specific digestion analysis of 45 non-IDD M and 10 cases of IDDM HLA-DQA_1, HLA-DQB_1 gene exon 2 exons. 10 cases of IDDM were all before the age of 20 onset, duration of 3 years or more, have ketoacidosis. Control were voluntary blood donors of the same age.