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目的探讨应用荧光定量聚合酶链反应(FQ-PCR)法检测男性乙肝患者血清和精液中HBV DNA含量的临床意义。方法选取男性乙肝患者70例,分别采集患者的精液和静脉血,并用FQ-PCR法检测精液及血清中HBV DNA含量。结果精液标本中HBV DNA阳性16例(占22.86%),HBV DNA含量为2.55×103～6.18×105IU/ml、平均为5.08×104IU/ml;血清标本中HBV DNA阳性62例(占88.57%),HBV DNA含量为1.21×103～7.52×107IU/ml、平均为9.40×105/ml。精液中HBV DNA含量与血清中HBV DNA含量呈正相关(r=0.743),且血清HBV DNA含量高于精液含量10倍左右;结论 FQ-PCR法定量检测精液中HBV DNA含量具有较强的特异性和灵敏度,为临床了解乙肝患者精液中HBV状态提供了帮助,具有重要的临床意义。
Objective To investigate the clinical significance of using fluorescent quantitative polymerase chain reaction (FQ-PCR) to detect HBV DNA in serum and semen of male patients with hepatitis B (HBV). Methods Seventy patients with hepatitis B were enrolled in this study. The semen and venous blood of patients were collected. The contents of HBV DNA in serum and semen were detected by FQ-PCR. Results The positive HBV DNA in semen samples were 16 (22.86%) and HBV DNA contents were 2.55 × 103 ~ 6.18 × 105 IU / ml with an average of 5.08 × 104IU / ml. The serum HBV DNA positive samples were 62 (88.57% , HBV DNA content of 1.21 × 103 ~ 7.52 × 107IU / ml, with an average of 9.40 × 105 / ml. The content of HBV DNA in seminal fluid was positively correlated with the level of HBV DNA in serum (r = 0.743), and the content of serum HBV DNA was 10 times higher than that of semen. Conclusion The quantitative detection of HBV DNA in seminal fluid by FQ-PCR method is more specific And sensitivity for the clinical understanding of hepatitis B patients semen in HBV status has provided help, has important clinical significance.