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确证植物叶绿体中存在遗传物质DNA(一般称CtDNA)及其相应基因表达系统的时间是本世纪六十年代(4、21),从此证实了早在本世纪初Correns等人根据某些影响叶绿体发育的因子的非孟德尔遗传方式所得的叶绿体中可能存在某些特殊的遗传因子的推论。以后的二十年间,叶绿体基因的研究如雨后春笋,不仅建立了从叶绿体中有效地分离和纯化ctDNA的方法,且对于它的一系列性状(包括理化性质、大小、基因定位等)和相应的遗传特性(如与细胞质雄不育的关系)进行了比较深入的研究。
Confirming the presence of plant genetic material in the chloroplast DNA (commonly referred to as CtDNA) and its corresponding gene expression system time is the sixties of this century (4,21), which confirmed that as early as the beginning of this century, Correns, who according to some of the impact of chloroplast development The corollaries derived from non-Mendelian inheritance may be corroborated by the presence of certain specific genetic elements in the chloroplast. In the next two decades, the research of chloroplast gene has sprung up not only to establish a method of effectively separating and purifying ctDNA from chloroplast, but also to analyze a series of traits (including physicochemical properties, size, gene location, etc.) Characteristics (such as the relationship with cytoplasmic male sterility) conducted a more in-depth study.