论文部分内容阅读
目的：观察平阳霉素诱发的大鼠白内障模型晶体上皮细胞ＤＮＡ损伤及修复的动态过程。方法：单细胞电泳法（Ｓｉｎｇｌｅｃｅｌｌｇｅｌａｓｓａｙ，ＳＣＧ）。结果：首次注射平阳霉素１２ｈ即出现严重的ＤＮＡ单链断裂（ＤＮＡｓｉｎｇｌｅｓｔｒａｎｄｂｒｅａｋｓ，ＳＳＢ），直至白内障产生，ＳＳＢ仍很严重，该ＤＮＡ损伤较其它模型更严重，其晶体囊内容物在致障过程中出现明显的液化与崩解。结论：平阳霉素性白内障模型在致障过程中有较其它模型更严重的ＤＮＡ单链损伤，且可能有不尽相同的机理存在。
Objective: To observe the dynamic process of DNA damage and repair of lens epithelial cells induced by Pingyangmycin in rat cataract model. Methods: single cell electrophoresis (Singlecellgelassay, SCG). Results: After the first injection of pingyangmycin, severe DNA single strand breaks (SSBs) appeared, and until the cataract was produced, the SSB was still serious. The DNA damage was more serious than other models. The content of the crystalline capsule in the barrier process Appear obvious liquefaction and disintegration. CONCLUSION: Pingyangmycin cataract model has more severe single-strand DNA damage than other models in the process of catastrophe, and may have different mechanisms.