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目的:研究不同浓度肿瘤坏死因子-α(TNF-α)对体外培养人牙囊细胞凋亡的影响。方法:第五代HDFCs分别与浓度为0(对照组)、50、100、200 ng/mL的TNF-α共孵育24 h,流式细胞仪AnnexinⅤ-FITC/PI双染技术检测牙囊细胞凋亡细胞比率。结果:HDFCs的凋亡率随TNF-α浓度升高而上升,由对照组的2.3%增加到7.1%。结论:TNF-可诱导HDFCs的凋亡,这种作用可能在牙齿发育、萌出过程中起重要作用。
Objective: To study the effects of different concentrations of tumor necrosis factor-α (TNF-α) on the apoptosis of human dental follicle cells in vitro. METHODS: The fifth generation HDFCs were incubated with TNF-α at concentrations of 0 (control group), 50,100,200 ng / mL for 24 h, respectively. The apoptosis of dental follicle cells was detected by flow cytometry AnnexinⅤ-FITC / PI double staining Dead cell ratio. Results: The apoptosis rate of HDFCs increased with the increase of TNF-α concentration from 2.3% to 7.1% in the control group. Conclusion: TNF- can induce the apoptosis of HDFCs, which may play an important role in the process of tooth development and eruption.