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为探讨不同组织细胞对化学致癌物诱导的程序外DNA合成的敏感性,本研究用放射自显影法观察了6种化合物在LACA小鼠多组织内诱导的程序外DNA合成。N—甲基—N’—硝基—N—亚硝基肌(MNNG)直接与小组织块和~3H-胸腺嘧啶核苷(~3H-TdR)体外培养;其它5种化合物二甲基亚硝胺(DMN)、4-硝基喹啉-1-氧化物(4NQO)、盐酸氮芥(NH_2·HCl)、膦氧氮丙啶(MAPO)、甲基苄基亚硝胺(NMBzA)通过不同途径体内给药作用后,取组织在体与~3H-TdR共同培养,冰冻切片,用放射自显影法观察组织细胞内程序
In order to investigate the sensitivity of different tissue cells to the process-induced DNA synthesis induced by chemical carcinogens, the extracellular DNA synthesis induced by the 6 compounds in LACA mice was observed by autoradiography. N-Methyl-N’-Nitro-N-Nitrosine (MNNG) was directly cultured with small tissue blocks and ~3H-thymidine (~3H-TdR); five other compounds were dimethyl Asia. Nitramine (DMN), 4-nitroquinoline-1-oxide (4NQO), hydrochloric acid mustard (NH_2 · HCl), phosphine oxygen aziridine (MAPO), methylbenzyl nitrosamine (NMBzA) passed After in vivo administration of different pathways, tissues were incubated in vivo with ~3H-TdR, cryosectioned, and observed by intracellular autoradiography.