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借助１５年的定位观测资料，系统地研究了长期施肥对土壤微生物含量、腐殖质组分、作物产量和土壤供肥能力的影响。结果表明，长期施用化肥和有机肥或两者配施，耕层土壤微生物和胡敏酸、富里酸、胡敏素含量增加，ＨＡ／ＦＡ比值增大；与腐殖质组成成分最为密切的农化性状是全氮和碱解氮，其次是速效磷；长期不施肥、单施氮肥或有机肥，因养分单一，供肥不足，作物产量逐年下降，抗逆力差。
With 15 years of observation data, the effects of long-term fertilization on soil microbial composition, humus composition, crop yield and soil fertility were systematically studied. The results showed that the contents of soil microbe and humic acid, fulvic acid and humin increased with the application of chemical fertilizers and organic fertilizers or both, and the ratio of HA / FA increased. The agrochemical characters most closely related to the components of humus were all Nitrogen and alkaline hydrolysis of nitrogen, followed by available phosphorus; long-term non-fertilization, nitrogen or organic fertilizer alone, due to a single nutrient, insufficient supply of crops, crop production declined year by year, the anti-adverse.