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目的观察蛋白酪氨酸磷酸酶1B(PTP1B)及Sam68(Src-associated substrated during mitosis of 68KD)在结直肠癌的发生发展中的表达及其相关性,探究其与结直肠癌临床病理特征的关系。方法采用免疫组织化学(SABC)法检测53例结直癌组织及20例癌旁正常组织中PTP1B、Sam68的表达情况,并结合临床病理学资料进行统计分析;采用Western blotting方法检测12例结直肠癌组织及对应癌旁正常组织中的PTP1B及Sam68蛋白表达情况。结果免疫组化结果显示PTP1B、Sam68在结直肠癌组织的阳性表达率明显高于正常大肠组织,差异均有统计学意义(均P<0.001)。PTP1B、Sam68的表达均与分化程度、TNM分期及淋巴结转移相关(P<0.05);相关性分析显示,PTP1B与Sam68表达呈正相关(r=0.463,P<0.001)。Western blotting检测结果显示结直肠癌组织中PTP1B和Sam68的蛋白表达量明显高于癌旁正常组织(P<0.05)。结论 PTP1B、Sam68可能与大肠癌的发生、浸润及转移有关,两者共同促进大肠癌的发生、发展、侵袭及转移。
Objective To investigate the expression and correlation of PTP1B and Sam68 (Src-associated substrated during mitosis of 68KD) in the development and progression of colorectal cancer and explore its relationship with the clinicopathological features of colorectal cancer . Methods The expression of PTP1B and Sam68 in 53 cases of colorectal cancer tissues and 20 cases of adjacent normal tissues were detected by immunohistochemistry (SABC) method. The expressions of PTP1B and Sam68 were analyzed by clinicopathological data. The expressions of PTP1B and Sam68 were detected by Western blotting in 12 cases of colorectal The expression of PTP1B and Sam68 protein in cancer tissue and corresponding normal tissues. Results The immunohistochemical results showed that the positive rates of PTP1B and Sam68 in colorectal cancer tissues were significantly higher than those in normal colorectal tissues (all P <0.001). The expression of PTP1B and Sam68 were correlated with the degree of differentiation, TNM stage and lymph node metastasis (P <0.05). Correlation analysis showed that there was a positive correlation between PTP1B and Sam68 expression (r = 0.463, P <0.001). The results of Western blotting showed that the protein expression of PTP1B and Sam68 in colorectal cancer tissues was significantly higher than that in adjacent normal tissues (P <0.05). Conclusions PTP1B and Sam68 may be involved in the carcinogenesis, invasion and metastasis of colorectal cancer. Both of them may promote the occurrence, development, invasion and metastasis of colorectal cancer.