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为人白细胞介素-10（hIL-10）的理论研究和临床应用研究提供材料。方法：利用基因工程技术，将hIL-10cDNA克隆在大肠杆菌表达载体中，在大肠杆菌中高效表达含凝血酶识别序列的hIL-10的融合蛋白。表达蛋白经凝酶消化后，可去除MS2细菌蛋白。结果：获得高纯度的非融合型rhIL-10。结论：活性分析表明rhIL-10具有抑制LPS刺激的外周血单个核细胞产生IL-6的能力。
Human interleukin-10 (hIL-10) theoretical research and clinical application of research materials. Methods: The hIL-10 cDNA was cloned into E. coli expression vector and the fusion protein of hIL-10 with thrombin recognition sequence was highly expressed in E. coli. After the protein is digested by the enzyme, MS2 bacterial protein can be removed. Results: High purity non-fusion rhIL-10 was obtained. CONCLUSION: The activity analysis shows that rhIL-10 has the ability to inhibit the production of IL-6 by LPS-stimulated peripheral blood mononuclear cells.