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目的:观察舒洛地特对血管紧张素Ⅱ(AngⅡ)诱导足细胞podocalyxin表达的影响,探讨舒洛地特降低蛋白尿的分子机制。方法:小鼠永生化足细胞传代培养,分为对照组、舒洛地特(30 LSU·ml~(-1))组、AngⅡ(10~(-8)mol·L~(-1))刺激组和AngⅡ+舒洛地特共孵育组,免疫荧光法检测podocalyxin在足细胞的表达和分布,RT-PCR和Western-blotting法检测podocalyxin的mRNA和蛋白表达。结果:AngⅡ刺激后podocalyxin胞膜和胞浆表达明显减少,podocalyxin mRNA(0.35±0.10比0.62±0.11)和蛋白(0.49±0.18比0.82±0.26)较对照组显著降低(P<0.05),舒洛地特共孵育后podocalyxin mRNA(0.59±0.12比0.35±0.10)和蛋白(0.70±0.17比0.49±0.18)较AngⅡ组显著增加(P<0.05)。结论:舒洛地特可抑制AngⅡ诱导的足细胞podocalyxin表达降低,修复足细胞电荷屏障。
OBJECTIVE: To observe the effect of sulodentia on the expression of podocalyxin in podocytes induced by angiotensin Ⅱ (Ang Ⅱ), and to explore the molecular mechanism of sulodentia in reducing proteinuria. Methods: The immortalized podocytes were subcultured and divided into control group (30 LSU · ml -1), Ang Ⅱ (10 -8 mol·L -1) Stimulated group and AngⅡ + sulodexide group. The expression and distribution of podocalyxin in podocytes were detected by immunofluorescence. The mRNA and protein expression of podocalyxin were detected by RT-PCR and Western-blotting. Results: The expressions of podocalyxin mRNA and protein in the cytoplasm and membrane of podocalyxin group were significantly lower than those of the control group (P <0.05). The levels of podocalyxin mRNA (0.35 ± 0.10 vs 0.62 ± 0.11) and protein (0.49 ± 0.18 vs 0.82 ± 0.26) Compared with AngⅡ group, podocalyxin mRNA (0.59 ± 0.12 vs 0.35 ± 0.10) and protein (0.70 ± 0.17 vs 0.49 ± 0.18) increased significantly (P <0.05). CONCLUSION: Schulutide inhibits the decrease of podocalyxin expression induced by AngⅡ in podocytes and repairs podocyte charge barrier.