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采用RT-PCR的方法,从茉莉酸处理的玉米叶片中克隆了oxylipins信号途径的3个关键调控基因(ZmAOS,ZmAOC和ZmHPL)cDNA片段,并根据同源性比较,获得了全长cDNA.基因表达分析表明,甜菜夜蛾危害可以诱导oxylipins信号途径关键基因的表达,且与外源茉莉酸甲酯的诱导作用相似;虫害对玉米未受损伤叶片防御相关基因(主要是一些调节植物防御物质合成的基因)有明显诱导作用,且与外源茉莉酸处理叶片的表达特性极为相似;先用茉莉酸信号途径的抑制剂SHAM处理玉米后,虫害不能诱导玉米防御基因的表达;外源绿叶性气味物质(GLVs)及β-罗勒烯处理对玉米防御相关基因的表达有明显诱导作用,同时外源GLVs及β-罗勒烯处理可以诱导茉莉酸信号途径关键调控基因的表达,但不能诱导ZmHPL基因的表达;用茉莉酸信号途径的抑制剂处理玉米后,GLVs处理对玉米防御基因表达的诱导作用明显降低,说明oxylipins信号途径在虫害诱导玉米产生化学防御的过程中起着关键作用,茉莉酸是其中重要的内源信号物质,而另一类oxylipins(GLVs)则作为外部的信号物质在虫害诱导植物产生系统性防御过程中起信息传递作用,且这种外部的信号物质部分是通过茉莉酸信号途径起作用.
The cDNA of three key regulatory genes (ZmAOS, ZmAOC and ZmHPL) of oxylipins signal pathway was cloned from jasmonic acid-treated corn by RT-PCR and the full-length cDNA was obtained according to homology comparison. The expression analysis showed that the damage induced by beet armyworm can induce the expression of key genes of oxylipins signaling pathway and is similar to the induction of exogenous jasmonate methyltransferase. Insect pests play an important role in the defense of uncorrupted maize leaves, mainly related to the regulation of plant defense substances Of the genes) and induced by exogenous jasmonic acid treatment leaves were very similar; first with jasmonic acid signal pathway inhibitor SHAM maize, the pest can not induce the expression of maize defensive genes; exogenous leafy smell Substance (GLVs) and β-Ocimesene treatment significantly induced the expression of defense-related genes in maize, while the treatment of exogenous GLVs and β-Ocimesene induced the expression of key regulatory genes of jasmonic acid signal pathway, but not the induction of ZmHPL gene After treatment with corn jasmonic acid signal inhibitor, the induction of GLVs on defense gene expression in maize significantly decreased The oxylipins signal pathway plays a key role in pest-induced chemical defenses in maize. Jasmonic acid is one of the important endogenous signal substances, while the other class of oxylipins (GLVs) acts as an external signaling substance in pest-induced plants to produce systemic The defensive process plays a role of information transfer, and this external signal substance partly functions through the jasmonate signaling pathway.