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本文采用顶空气相色谱技术建立了一种快速评价抗菌纸抑菌性的方法,主要是通过检测在规定的培养过程中消耗的氧气量或生成的二氧化碳量来反映细菌的生长程度,从而实现对抗菌纸的抑菌性进行评价。结果表明:在37℃、培养时间6h-8h、大肠杆菌菌液浓度为2.9×10~2CFU/m L、培养液体积为5m L、培养容器为顶空瓶的条件下,最适宜氧气和二氧化碳信号的检测,并可简便准确计算出抑菌率值。本方法与传统方法(48h-72h)相比,大大提高了检测的效率,适用于对抗菌纸抑菌性的快速评价。
In this paper, a rapid method for the evaluation of antibacterial paper antibacterial paper was established by headspace gas chromatography, mainly by measuring the amount of oxygen consumed or the amount of carbon dioxide generated in the process of culture to reflect the growth of bacteria, Antibacterial antibacterial paper was evaluated. The results showed that under the conditions of 37 ℃, culture time 6h-8h, the concentration of Escherichia coli bacteria was 2.9 × 10 ~ 2CFU / m L, the volume of culture medium was 5m L and the culture vessel was a headspace bottle, the most suitable oxygen and carbon dioxide Signal detection, and can easily and accurately calculate the antimicrobial value. Compared with the traditional method (48h-72h), the method greatly improves the detection efficiency and is suitable for the rapid evaluation of antibacterial paper antibacterial properties.