建立了基质金属蛋白酶-1的活性测定方法及临床应用的部分意义。基质金属蛋白酶是广泛分布于细胞外基质等部位的蛋白酶类,目前已知有14种,基质金属蛋白酶-1主要水解作用于细胞外基质的Ⅰ,Ⅱ,Ⅲ型胶原蛋白,其最适底物为Ⅰ型胶原。该研究采用从胎牛皮肤分离提取Ⅰ型胶原蛋白,并经HPLC、电泳鉴定后,以~3H-乙酸酐标记作为放 Established the determination of MMP-1 activity and its clinical significance. Matrix metalloproteinases are widely distributed in the extracellular matrix and other proteases, there are currently known 14 kinds of matrix metalloproteinase -1 major hydrolysis of the extracellular matrix type Ⅰ, Ⅱ, Ⅲ collagen, the most suitable substrate Type I collagen. In this study, type I collagen was isolated from fetal bovine skin and identified by HPLC and electrophoresis. The 3H-acetic anhydride was used as a marker