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在一些基层医院病理科技术室硬件配备往往不足,这给制片工作带来一定难度和困难,尤其在寒冷季节当室温低于10℃时,没有空调及组织脱水机或当脱水机故障时组织块脱水往往较难脱好,这样就影响制片质量。笔者经过实践采用把组织块脱水缸放在摊片机的摊片水槽内进行加温水浴脱水。 1 器材准备 摊片机一台 2 具体方法与效果 按常规切取组织块在10％甲醛液中固定过夜,次日上午将组织块水洗后置75％乙醇缸中加盖放入恒温50℃摊片机槽内30分钟,再置于95％乙醇(两道)各1小时,再投入无水乙醇中1小时,无水乙醇
In some grassroots hospitals, pathology and technical room hardware is often inadequate, which brings some difficulties and difficulties in the preparation work, especially in the cold season when the room temperature is below 10 ℃, there is no air conditioning and tissue dehydration machine or when the dehydration machine failure Block dehydration is often more difficult to take off, thus affecting the quality of production. I practice the use of the tissue block dewatering tank stall on the stalls in the water tank to heat the water bath dehydration. A device to prepare a plate tester a 2 specific methods and effects according to conventional cutting tissue block fixed in 10% formaldehyde solution overnight, the next day after washing the tissue block washed 75% ethanol cylinder stamped into a constant temperature 50 ° C stalls Machine slot for 30 minutes, and then placed in 95% ethanol (two) each 1 hour, and then into absolute ethanol for 1 hour, absolute ethanol