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A novel fluorimetric method for determination of laccase activity in organic solvents is proposed, based on the oxidation or o-phenylenediamine (1, 2-diaminobenzene, OPDA) catalyzed by laccaseyielding 2,3-diaminopkenazine. The optimal conditions for laccase in organic media are T=55℃, pH=6.5,1.0×10-2mol/L OPDA, 1. 25 mL etkanol, 1.25 mL1,4-dioxane and 1. 25 mL acetone. The linear range ofthe method proposed in ethanol, 1,4-dioxane and acetoue media were 0. 44~19. 33, 0. 11~20. 85, 0. 38~21. 05 U with the detection limit of 0. 088, 0. 022, 0. 076 U, respectively. The proposed method has been anplied to the analysis of laccase activity of real samples with more accurate and sensitive than that of the previous method reported.
A novel fluorimetric method for determination of laccase activity in organic solvents is proposed, based on the oxidation or o-phenylenediamine (1,2-diaminobenzene, OPDA) catalyzed by laccaseyielding 2,3-diaminopkenazine. The optimal conditions for laccase in organic media are T = 55 ° C, pH = 6.5, 1.0 × 10 -2 mol / L OPDA, 1.25 mL etkanol, 1.25 mL 1,4-dioxane and 1.25 mL acetone. The linear range of the method proposed in ethanol, dioxane and acetoue media were 0.44 ~ 19.33, 0.11 ~ 20.85, 0.38 ~ 21. 05 U with the detection limit of 0. 088, 0. 022, 0. 076 U, respectively. The proposed method has been an applied to the analysis of laccase activity of real samples with more accurate and sensitive than that of the previous method reported.