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杂种优势的利用使得水稻产量较半矮杆常规稻增加了 1 5%以上 ,为了保持产量的稳定 ,杂交稻品种还应具备对病虫的可遗传抗性 ,比如对茎杆钻心虫的抗性。而来自于苏云金杆菌并经人工剪裁合成的CryIA(b)基因便是有效地抗茎杆钻心虫基因之一。实践证明 ,基因转化是一项导入细菌基因于水稻基因组的行之有效的技术。笔者已利用获得转基因保持系这一途径来选育抗虫的杂交稻。利用基因枪方法 ,将CryIA(b)基因以及驱动该基因的 3 5S启动子成功地转入了IRRI的骨干保持系IR68899B。该基因的整合和表达已由Southern和Western杂交所证明。目前已分析到转基因植株的第二世代 (T2 )。鉴定表明 ,Bt+转基因植株中黄色茎杆钻心虫的抗性明显提高。本文首次报导转基因保持系的获得 ,以用于杂交稻品种的选育
The utilization of heterosis makes the yield of rice increase by more than 15% compared with that of conventional dwarf rice. In order to maintain the stable yield, the hybrid rice should have heritable resistance to pests such as resistance to stem borer . The CryIA (b) gene from Bacillus thuringiensis, which has been artificially tailored, is one of the genes responsible for stem-borer resistance. It has been proved that gene transformation is an effective technique to import bacterial genes into rice genome. I have used this approach to obtain transgenic lines to breed insect-resistant hybrid rice. The CryIA (b) gene and the 35S promoter driving the gene were successfully transferred into IR68 backbone backbone IR68899B by gene gun method. The integration and expression of this gene has been demonstrated by Southern and Western blotting. The second generation of transgenic plants has been analyzed (T2). Identification showed that resistance of yellow stem borer was significantly increased in Bt + transgenic plants. This paper reports for the first time the availability of transgenic maintainers for the breeding of hybrid rice varieties