论文部分内容阅读
目的:通过观测低氧环境对体外培养大鼠颏舌肌成肌细胞分化及低氧诱导因子1α(HIF-1α)表达的影响,探讨低氧引起颏舌肌损伤的机制以及HIF-1α在其中的作用。方法:根据环境氧浓度不同,将体外培养的原代大鼠颏舌肌成肌细胞分为正常氧浓度组(NC)(21%)和低氧组(HG)(1%),分别诱导分化0 d、1 d、3 d、6 d。采用RT-PCR及Western blotting检测生肌调节因子(MyoD)、肌源性决定因子(myogenin)、肌球蛋白重链(MHC)以及HIF-1α的mRNA及蛋白表达;倒置显微镜下观察成肌细胞分化的形态变化。结果:在颏舌肌成肌细胞分化过程中,两种氧状态下HIF-1αmRNA表达均没有显著变化(P>0.05),但蛋白表达逐渐上调;低氧对MyoD、myogenin、MHC的mRNA(P<0.05)和蛋白表达有显著的抑制作用,致肌管形成延迟;低氧使HIF-1αmRNA(P<0.05)和蛋白显著上调。结论:低氧环境可能是通过上调HIF-1α表达抑制大鼠颏舌肌成肌细胞的分化,从而抑制颏舌肌损伤的修复。
OBJECTIVE: To investigate the effects of hypoxia on the differentiation of myogenic myoblasts and the expression of hypoxia-inducible factor 1α (HIF-1α) in vitro and to explore the mechanism of hypoglycemia-induced genioglossus injury and the role of HIF-1α in it Role. Methods: Primary cultured rat genioglossus myoblasts were divided into normal oxygen concentration (NC) group (21%) and hypoxia group (HG) (1%) according to the different ambient oxygen concentrations. 0 d, 1 d, 3 d, 6 d. The mRNA and protein expressions of MyoD, myogenin, myosin heavy chain (MHC) and HIF-1α were detected by RT-PCR and Western blotting. The myoblasts were observed under inverted microscope Differentiation of morphological changes. Results: During the process of genioglossal myogenic differentiation, there was no significant change in the expression of HIF-1α mRNA (P> 0.05), but the expression of HIF-1α was up-regulated. The mRNA and protein expressions of MyoD, myogenin and MHC <0.05) and protein expression, delayed myotube formation. Hypoxia significantly upregulated the expression of HIF-1αmRNA (P <0.05) and protein. CONCLUSION: Hypoxic environment may inhibit the repair of genioglossus muscle injury by up-regulating the expression of HIF-1α in the genioglial myoblasts.