液相色谱中再循环(Recycle)技术在改进凝胶色谱低柱容量上首先得到应用。这种技术将一次分离后样品从检测器出口再返回色谱柱入口,在同一色谱柱内连续多次循环分离。在不增加柱压和柱长条件下,提高了柱样品容量和分离度。本文导出再循环制备分离中最佳循环次数和最大分离样品体积计算公式,以制备分离蓝科植物荚果香蓝豆中天然香蓝素的 Recycling technology in liquid chromatography was first applied to improve the low column capacity of gel chromatography. This technique separates a single sample from the detector outlet and back to the column inlet and continues to cycle multiple times in the same column. Increasing column sample capacity and resolution without increasing column pressure and column length. This paper derives the optimal number of cycles for recirculation in preparative separation and the formula for calculating the maximum separated sample volume for the preparation of natural alcocyanin isolated from the blueberry plant