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目的探讨抗环瓜氨酸肽抗体(抗CCP抗体)检测对类风湿关节炎(RA)诊断的意义。方法采用酶联免疫吸附试验(ELISA)检测115份人血清的抗CCP抗体,同时采用免疫透射比浊法定量检测类风湿因子(RF),包括40例RA患者,45例其它风湿病患者,30名正常人;并分析抗CCP抗体与RF实验结果之间的相关性。结果在40例RA病人中,抗CCP抗体的阳性率为80.0%,在其它风湿病人中的阳性率为7.0%,抗CCP抗体对RA的敏感性和特异性分别为80.0%、96.0%,其敏感性高于RF,但差异无统计学意义(P>0.05),特异性明显高于RF(P<0.05)。联合应用抗CCP抗体与RF进行诊断,二者均阳性时敏感性为65.0%,特异性为97.3%。抗CCP抗体与RF实验结果之间无相关性。结论抗CCP抗体对RA具有较好的敏感性和很高的特异性,可与RF相互补充,联合检测可提高对RA早期诊断的准确性。
Objective To investigate the diagnostic value of anti-cyclic citrullinated peptide antibody (anti-CCP antibody) in the diagnosis of rheumatoid arthritis (RA). Methods Serum anti-CCP antibodies were detected by enzyme-linked immunosorbent assay (ELISA) in 115 individuals. Rheumatoid factor (RF) was quantitatively detected by immunoturbidimetric assay, including 40 patients with RA, 45 patients with other rheumatic diseases, 30 Normal; and analyzed the correlation between anti-CCP antibody and RF experimental results. Results The positive rate of anti-CCP antibody was 80.0% in 40 RA patients and 7.0% in other rheumatic diseases. The sensitivity and specificity of anti-CCP antibody to RA were 80.0% and 96.0%, respectively The sensitivity was higher than RF, but the difference was not statistically significant (P> 0.05), the specificity was significantly higher than RF (P <0.05). The combination of anti-CCP antibody and RF diagnosis, both were positive when the sensitivity was 65.0%, the specificity was 97.3%. There was no correlation between anti-CCP antibody and RF test results. Conclusion Anti-CCP antibody has good sensitivity and specificity for RA and can be complemented with RF. Combined detection can improve the accuracy of early diagnosis of RA.