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蛋白水解酶以及某些化学试剂如溴化氢、羟胺等具有专一性水解肽键的能力.这极大地方便了蛋白质的序列测定以及其结构与功能的研究.然而,有限的蛋白水解酶远远不能满足科研和应用开发的需要.因此,人工蛋白水解酶的研究近年来正日益受到关注.由于某些蛋白水解酶如羧肽酶和氨肽酶等是以金属离子作为活性中心活化肽键的,人工金属配合物蛋白水解酶的研究不仅可以扩大水解肽键试剂的种类,而且也为深入了解蛋白水解酶的反应机理提供依据.近年来,我们发现某些Pd(Ⅱ)配合物具有高效和选择性地水解多肽和蛋
Proteolytic enzymes, and certain chemical agents such as hydrogen bromide, hydroxylamine and the like have the ability to specifically hydrolyze peptide bonds, which greatly facilitates the sequencing of proteins and their structure and function. However, limited proteolytic enzymes Far from being able to meet the needs of scientific research and application development.Therefore, the research of artificial proteolytic enzyme is getting more and more attention in recent years.As certain proteolytic enzymes such as carboxypeptidase and aminopeptidase are metal ions as the active center to activate the peptide bond , The study of artificial metal complex proteolytic enzymes not only can broaden the types of hydrolyzed peptide bond reagents, but also provide a basis for understanding the reaction mechanism of proteolytic enzymes.In recent years, we have found that some Pd (Ⅱ) complexes have high efficiency And selectively hydrolyze polypeptides and eggs